DPP4 antibody

Catalog No. ABIN967645

This Mouse Monoclonal antibody specifically detects DPP4 in WB, FACS, IP and IHC (fro). It exhibits reactivity toward Rat and has been mentioned in 8+ publications.

Quick Overview for DPP4 antibody (ABIN967645)

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This DPP4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: OX-61

Product Details anti-DPP4 Antibody

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat dendritic cells enriched from thoracic duct lymph

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-DPP4 antibody (ABIN967645)

Iwaki-Egawa, Watanabe, Fujimoto: "CD26/dipeptidyl peptidase IV does not work as an adenosine deaminase-binding protein in rat cells." in: Cellular immunology, Vol. 178, Issue 2, pp. 180-6, (1997) (PubMed).

Johnson, Zhu, Augustin-Voss, Pauli: "Lung endothelial dipeptidyl peptidase IV is an adhesion molecule for lung-metastatic rat breast and prostate carcinoma cells." in: The Journal of cell biology, Vol. 121, Issue 6, pp. 1423-32, (1993) (PubMed).

Kameoka, Tanaka, Nojima, Schlossman, Morimoto: "Direct association of adenosine deaminase with a T cell activation antigen, CD26." in: Science (New York, N.Y.), Vol. 261, Issue 5120, pp. 466-9, (1993) (PubMed).

Bristol, Sakaguchi, Appella, Doyle, Takács: "Thymocyte costimulating antigen is CD26 (dipeptidyl-peptidase IV). Costimulation of granulocyte, macrophage, and T lineage cell proliferation via CD26." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 367-72, (1992) (PubMed).

Gorrell, Wickson, McCaughan: "Expression of the rat CD26 antigen (dipeptidyl peptidase IV) on subpopulations of rat lymphocytes." in: Cellular immunology, Vol. 134, Issue 1, pp. 205-15, (1991) (PubMed).

McCaughan, Wickson, Creswick, Gorrell: "Identification of the bile canalicular cell surface molecule GP110 as the ectopeptidase dipeptidyl peptidase IV: an analysis by tissue distribution, purification and N-terminal amino acid sequence." in: Hepatology (Baltimore, Md.), Vol. 11, Issue 4, pp. 534-44, (1990) (PubMed).

Piazza, Callanan, Mowery, Hixson: "Evidence for a role of dipeptidyl peptidase IV in fibronectin-mediated interactions of hepatocytes with extracellular matrix." in: The Biochemical journal, Vol. 262, Issue 1, pp. 327-34, (1989) (PubMed).

Hanski, Huhle, Gossrau, Reutter: "Direct evidence for the binding of rat liver DPP IV to collagen in vitro." in: Experimental cell research, Vol. 178, Issue 1, pp. 64-72, (1988) (PubMed).

Target Details for DPP4

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Alternative Name: CD26

Background: The OX-61 antibody reacts with a type II transmembrane glycoprotein, CD26, which is a serine exoprotease identified as dipeptidyl/peptidase IV. Among various biological activities, rat CD26 binds fibronectin and collagen. Rat CD26 is involved in the costimulation of thymocyte proliferation in vitro, particularly the CD4-/CD8- subset, and is developmentally regulated on hematopoietic cells. Although mouse and human CD26 anchor ADA (adenosine deaminase) to cell membranes, rat CD26 does not function as an ADA-binding protein. Rat CD26 is expressed in lung endothelial cells, as well as in various epithelial cells. T cells express lower levels of CD26 than CD4-/CD8- thymocytes. The distribution of CD26 antigen in rat bone marrow cells is similar to that of human CD26. OX-61 monoclonal antibody stains CD4+, CD8+, and Ig+ lymphocytes, and the staining increases upon activation.

Pathways: Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity